Cell transplantation models / T24

T24 cell transplantation models

Bladder carcinoma

Human bladder TCC line. s.c. locks Ramakrishnan 5×10⁶ in 100 μL HBSS:Matrigel 50:50; parental bladder dose not locked. Do not copy 5637 or 231.

SKU: MC-h208 · Disease models · In-vitro spheroid

Protocols(2)

T24 subcutaneous xenograft

Human bladder TCC T24 s.c. CDX. This SOP uses Ramakrishnan: 5×10⁶ in 100 μL HBSS:Matrigel 50:50, nude. That paper’s HT1376 is 2×10⁶ and UM-UC-3 is 3×10⁶—neither is T24. Not intramural/intravesical orthotopic bladder. Do not copy 5637’s 3×10⁶ / 0.2 mL PBS or MDA-MB-231’s 2×10⁶ / 100 μL.

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T24 orthotopic bladder

Intramural or intravesical bladder for local growth. Parental T24 default bladder cell number is not locked. Do not copy s.c. 5×10⁶ / 100 μL 50:50 or 5637.

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Protocol index

Red [Non-metastatic] = local tumorigenesis, not a distant-met endpoint; [Weak metastasis evidence] = poorly supported for parental line—exploratory/negative-control use.

Related

Disclaimer: Research use only (RUO). Not clinical guidance or a substitute for institutional animal SOPs. In vivo work requires ethics approval. Inline [n] maps to each section’s reference list.