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A: Antibodies should be stored at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Aliquoting is recommended.
A: Please choose appropriate solvent (DMSO, water, or buffer) according to the product manual and prepare stock solutions.
A: Optimize blocking conditions, reduce antibody concentration, and increase washing times.
A: Discard contaminated cells immediately, thoroughly clean the incubator, and check media and serum.