Melanoma couples MAPK lesions (BRAF/NRAS/NF1) with immune evasion; UV damage and clonal evolution shape heterogeneity. We provide differentiation and checkpoint-related antibodies plus BRAF/MEK probes for resistance, TME, and combination studies.
Focus areas: MAPK reactivation & bypass, primary/acquired IO resistance, TMB/neoantigens, brain metastasis models
Commonly used for mechanistic studies; follow your lab SOP, compound datasheets, and ethics approvals.
| Compound | Targets / pathways | Experimental notes |
|---|---|---|
| Vemurafenib | Selective BRAF V600 | MAPK probe; watch keratinocyte hyperplasia and bypass. |
| Dabrafenib | BRAF V600 | Often paired with MEKi; monitor fever/ocular toxicity. |
| Trametinib | MEK1/2 | Standard BRAFi partner; baseline cardio/ocular monitoring. |
| Cobimetinib | MEK1/2 | Alternative MEK partner; rash/diarrhea management. |
| Ipilimumab (anti-CTLA-4 mAb) | CTLA-4 | Strong immune activation; colitis/endocrine AEs need vigilance. |
| Pembrolizumab (anti-PD-1 mAb) | PD-1 | Efficacy varies with TMB/PD-L1; pair with immune infiltrate readouts. |
| Encorafenib + Binimetinib (combo) | BRAF + MEK | Clinical combo mapping; translate doses carefully in animals. |