Stem cells

Stem cell research spans self-renewal, multipotent differentiation, and regenerative applications. We provide antibodies and tools around pluripotency markers (Oct4/Sox2/Nanog) and signaling hubs (Wnt, TGF-β/BMP, FGF, Notch) for maintenance and lineage specification—from basic mechanisms to translation.

Focus areas: pluripotency networks & epigenetic resetting, lineage specification & fate switches, organoids & niche crosstalk, expansion analytics & QC markers

Stem-cell-related antibodies (curated)

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Hot topics & directions

  • Pluripotency maintenance vs exit: TF networks vs chromatin accessibility locking or releasing pluripotency.
  • Lineage specification & germ-layer allocation: Wnt/BMP/Nodal–Smad gradients encoding positional information and fate.
  • Adult stem cells & tissue homeostasis: Niche signaling and metabolic demands for MSC/HSC in disease or aging.
  • Organoids & regeneration models: Polarity, matrix mechanics, and paracrine feedback in 3D models.

Related targets

Core pluripotency network
POU5F1 (OCT4), SOX2, NANOG, KLF4, MYC, LIN28A
Surface & lineage markers (examples)
SSEA3/4, TRA-1-60/81, CD34, CD133 (PROM1), CD90 (THY1), NGFR
Culture maintenance & cycle
LIF–STAT3, FGF–ERK, TGFβ–SMAD, CDK inhibitors (context), p53 pathway
Morphogen axes
WNT/β-catenin, BMP/SMAD, SHH/GLI, NOTCH, NODAL/Activin

Key signaling pathways

  1. LIF–JAK–STAT3 (mouse PSC context)
    Central in mouse PSC maintenance; human PSCs rely more on FGF–TGFβ combinations.
  2. Wnt / β-catenin ↔ chromatin remodeling
    Couples renewal to lineage priming; agonist/antagonist dose sets renewal vs differentiation.
  3. TGF-β / Activin–SMAD2/3 vs BMP–SMAD1/5/8
    Opposing axes in germ-layer induction—interpret alongside medium formulation and timing.
  4. NOTCH–DLL/JAG lateral signaling
    Fate switches in neural/hematopoietic contexts; γ-secretase inhibition needs careful controls.

Tool compounds (research use)

Commonly used for mechanistic studies; follow your lab SOP, compound datasheets, and ethics approvals.

CompoundTargets / pathwaysExperimental notes
CHIR99021GSK3β / Wnt–β-catenin axis (concentration-dependent)Common in PSC maintenance/differentiation—tune with ligands and co-culture context.
SB431542ALK4/5/7 (TGF-β / Activin branch)Used in neural induction workflows; mind SMAD2/3 suppression and lineage bias.
PD0325901MEK1/2Component of 2i-like media; tightly window concentrations with CHIR, etc.
Y-27632ROCKImproves survival after dissociation; mind cytoskeletal/off-target differentiation effects.
DorsomorphinBMP type I receptors (ALK2/3/6)BMP inhibition in neural induction; check dose–response vs known off-targets.
Retinoic acid (RA)RAR/RXR (pro-differentiation)Classic pro-differentiation cue; light-sensitive—standardize storage/spiking.
CyclopamineSmoothened (Hedgehog pathway)Hh-dependent lineage decisions; mind toxicity and solubility vehicles.