Ubiquitination is a deeply conserved PTM that not only marks proteolytic substrates but also rewires signaling, DNA-damage responses, immune-receptor regulation, and chromatin states. We provide antibodies and chemical tools spanning Ub, E3 ligases, and DUBs—from linkage-specific recognition to E3–substrate engagement and PROTAC design.
Focus areas: linkage-encoded signaling (K48/K63/linear, etc.), E3 substrate recognition & phospho-coupling, DUB editing & signal termination, CRL/NEDD8 dynamics
Commonly used for mechanistic studies; follow your lab SOP, compound datasheets, and ethics approvals.
| Compound | Targets / pathways | Experimental notes |
|---|---|---|
| PYR-41 | UBA1 (E1 activating enzyme) | Blocks upstream Ub activation; cytotoxic/off-target—use dose/time matrices. |
| MLN4924 (Pevonedistat) | NAE1 / NEDDylation (CRL axis) | Common for CRL turnover/substrate accumulation; pair cell-cycle readouts. |
| P5091 | USP7 | DUB tool; intersects MDM2–p53/DDR—mind substrate spectrum. |
| PR-619 | Broad-spectrum DUB inhibition | Coarse DUB-dependency screens; follow with selective inhibitors or genetics. |
| MG132 | Proteasome | Ub-substrate accumulation vs turnover block; mind non-specific stress. |
| Lactacystin | Proteasome (irreversible tool) | Often paired vs MG132; cytotoxic—window doses. |
| Lenalidomide | CRBN (IMiD / molecular-glue context) | CRBN substrate recruitment/degradation contexts; species/cell-line dependent. |