Cellular jakStat · target hub

CNTFR

CNTFR (CNTFR)

CNTFR is a cytokine receptor on the JAK-STAT signaling pathway axis. Validate with UniProt and literature. Indexed under "IL-6 family / gp130" for antibodies and assay guidance.

Back to pathway overviewSearch: CNTFRUniProt (Q9Y243)

Research notes

  • Combine with module neighbors to test non-redundant roles of CNTFR.
  • Pair phospho/phenotypic readouts with stimulation and inhibitor controls.
  • Validate antibody clone and compartment; include KD/KO controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Ligand stimulationLigand-dependent phosphorylation
JAK couplingJAK recruitment and trans-phosphorylation
CN / expressionCopy number & tissue expression

Naming and prevalence vary—annotate clinically with authoritative databases.

Assay readouts for CNTFR

  • Receptor–JAK co-IP; ligand dependence.
  • Pair p-STAT downstream readouts.
  • Distinguish γc vs gp130 family context.
  • Primary immune cells vs tool lines.

CNTFR experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for CNTFR studies (examples)

  • Tool lines: Jurkat, TF-1, Ba/F3, HEK293T, HepG2—screen by CNTFR expression (CCLE/DepMap).
  • Combine with neighbors (IL6R, IL6ST, IL11RA, LIFR) via KD/pharmacology.
  • Overexpression/rescue in HEK293T supports mechanism and IP.
  • Isogenic/CRISPR models separate dependency from bypass.

[2] Cell samples for CNTFR Western blot

  • Whole-cell lysates; phosphatase inhibitors for phospho blots.
  • Optimize stimulation time courses per ligand/stress.
  • Controls: KD/KO or inhibitors.
  • BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for CNTFR Western blot

  • Matched tumor/adjacent frozen tissues.
  • Mouse models—mind species cross-reactivity.
  • Primary cells/PDCs when approved.
  • FFPE needs specialized extraction; frozen preferred for phospho.

[4] Cell samples for CNTFR immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-CNTFR.
  • Endogenous IP: high-expression lines.
  • Stimulation enriches interactions.
  • Controls: isotype IgG, empty vector, KO.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous CNTFR can differ in dosage, splicing, and compartmentation.

Quick search presets

CNTFRCNTFR antibodyJAK STAT antibody

CNTFR-related antibodies (keyword-biased)

Adds CNTFR keyword bias atop the JAK-STAT 信号通路 antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

IL6RIL6STIL11RALIFROSMR

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the cellular jakStat hub for neighboring targets—cross-check PI3K, MAPK, and transmembrane transport pages as needed.

Cellular jakStat·PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Transmembrane transport