Cellular jakStat · target hub

TYK2

TYK2 (TYK2)

TYK2 is a JAK kinase on the JAK-STAT signaling pathway axis. Validate with UniProt and literature. Indexed under "JAK family kinases" for antibodies and assay guidance.

Back to pathway overviewSearch: TYK2UniProt (Q9Y243)

Research notes

  • Combine with module neighbors to test non-redundant roles of TYK2.
  • Pair phospho/phenotypic readouts with stimulation and inhibitor controls.
  • Validate antibody clone and compartment; include KD/KO controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
ActivatingKinase/receptor activation
Fusion / amplificationFusion or amplification (cohort-dependent)
CN gainCopy gain and overexpression

Naming and prevalence vary—annotate clinically with authoritative databases.

Assay readouts for TYK2

  • p-JAK (Tyr1007/1008, isoform-dependent) and total TYK2.
  • Cytokine stimulation time courses; JAK inhibitor controls.
  • Receptor co-IP for complex assembly.
  • Downstream gene panels (project-dependent).

TYK2 experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for TYK2 studies (examples)

  • Tool lines: Jurkat, THP-1, HeLa, HEK293T, U937—screen by TYK2 expression (CCLE/DepMap).
  • Combine with neighbors (JAK1, JAK2, JAK3) via KD/pharmacology.
  • Overexpression/rescue in HEK293T supports mechanism and IP.
  • Isogenic/CRISPR models separate dependency from bypass.

[2] Cell samples for TYK2 Western blot

  • Whole-cell lysates; phosphatase inhibitors for phospho blots.
  • Optimize stimulation time courses per ligand/stress.
  • Controls: KD/KO or inhibitors.
  • BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for TYK2 Western blot

  • Matched tumor/adjacent frozen tissues.
  • Mouse models—mind species cross-reactivity.
  • Primary cells/PDCs when approved.
  • FFPE needs specialized extraction; frozen preferred for phospho.

[4] Cell samples for TYK2 immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-TYK2.
  • Endogenous IP: high-expression lines.
  • Stimulation enriches interactions.
  • Controls: isotype IgG, empty vector, KO.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous TYK2 can differ in dosage, splicing, and compartmentation.

Quick search presets

TYK2TYK2 antibodyJAK STAT antibody

TYK2-related antibodies (keyword-biased)

Adds TYK2 keyword bias atop the JAK-STAT 信号通路 antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

JAK1JAK2JAK3

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the cellular jakStat hub for neighboring targets—cross-check PI3K, MAPK, and transmembrane transport pages as needed.

Cellular jakStat·PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Transmembrane transport