PI3K/Akt/mTOR · target hub

PIK3C2A

Phosphatidylinositol 4-phosphate 3-kinase catalytic subunit type 2 alpha (PI3K-C2α)

PIK3C2A encodes the class II PI3K α isoform, converting PI(4)P (among inputs) to PI(3,4)P₂ at diverse membranes with roles in RTK-linked endocytosis/recycling, vesicle trafficking, and cytoskeletal/adhesion crosstalk—parallel to, but distinct from, class I PIP₃–Akt signaling; do not equate pathway readouts with plasma-membrane PIP₃ production.

Research notes

  • Pair endocytosis/recycling assays (e.g., transferrin/EGFR) and lipid probes to separate PI(3,4)P₂ / PI4P pools from class I PIP₃.
  • Use genetics/KD matrices versus PIK3C2B/PIK3C2G to map partially non-redundant roles across tissues and stimuli.
  • For Akt/mTOR-centric projects, annotate class I PI3K genotypes—class II PI3Ks often tune trafficking and signaling compartmentation.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Expression contextTissue/cell-type–dependent expression
Rare variantsSporadic variants—require functional validation
Pathway couplingCo-occurs with neighboring nodes on the hub

Naming and prevalence vary by cohort and assay—annotate clinically with COSMIC, ClinVar, OncoKB, and datasheets; research context only.

Assay readouts for PIK3C2A

  • Lipid side: PIP₃/PI(3,4)P₂ probes or PH-domain pulldowns—separate PIK3C2A from class II/III compartments.
  • Biochemistry: IP–kinase or membrane recruitment; co-IP regulatory partners.
  • Downstream: p-AKT Thr308/Ser473, p-S6K, p-4E-BP1—pair totals and inhibitor titrations.
  • Genetics: PTEN/INPP4 status; NGS/ddPCR for lesions and copy number.

PIK3C2A experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for PIK3C2A studies (examples)

  • Tool lines: HEK293T, MCF-7, HCT116, Jurkat, U937—screen by PIK3C2A expression and pathway context (CCLE/DepMap).
  • Combine with neighbors (PIK3C2B, PIK3C2G) via KD/pharmacology to test non-redundancy.
  • Overexpression/rescue in HEK293T supports mechanism and IP workflows.
  • Isogenic/CRISPR models separate pathway dependency from bypass survival.

[2] Cell samples for PIK3C2A Western blot

  • Whole-cell lysates—optimize RIPA/NP-40 per antibody; phosphatase inhibitors for phospho blots.
  • Stimulation: serum starvation ± insulin/EGF or amino-acid withdrawal/refeed as relevant.
  • Controls: siRNA/shRNA, CRISPR KO, or inhibitors to validate band specificity.
  • Loading: BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for PIK3C2A Western blot

  • Matched tumor/adjacent frozen tissues after pathology review.
  • Mouse GEMM or xenografts—mind species antibody cross-reactivity.
  • Primary cells/PDCs when ethically approved.
  • FFPE needs specialized extraction; frozen tissue preferred for phospho work.

[4] Cell samples for PIK3C2A immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-PIK3C2A for complex capture.
  • Endogenous IP: high-expression lines; often ≥1–5×10⁶ cells per IP.
  • Stimulation: ligand or nutrient treatments enrich interactions—pilot time courses.
  • Controls: isotype IgG, empty vector, KD/KO negatives.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous PIK3C2A can differ in dosage, splicing, and compartmentation—in organoids/PDX, record passage, matrix, and drug history.

Quick search presets

PIK3C2A-related antibodies (keyword-biased)

Adds PIK3C2A keyword bias atop the PI3K/Akt/mTOR antibody pool—if sparse, use presets above or global search.

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the PI3K/Akt/mTOR hub for neighboring nodes and assay guidance—cross-check MAPK, RTK, metabolism, and autophagy pages as needed.