PI3K/Akt/mTOR · target hub

PIK3C2B

Phosphatidylinositol 4-phosphate 3-kinase catalytic subunit type 2 beta (PI3K-C2β)

PIK3C2B encodes class II PI3K β with a Ras-binding region alongside PI3K/C2 modules; broadly expressed, it supports adhesion, motility, and receptor-driven membrane remodeling. Pharmacology overlaps imperfectly with class I PI3K inhibitors—phenotypes are highly context-specific.

Back to pathway overviewSearch: PIK3C2BUniProt (O00750)

Research notes

  • In motility/adhesion models, compare KD/rescue against PIK3C2A to test β-biased dependence.
  • When using legacy PI3K inhibitors, check isoform selectivity—do not collapse phenotypes onto PIP₃–Akt alone.
  • Pair endosomal/plasma-membrane markers (super-resolution where helpful) to map compartmental outputs.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Expression contextTissue/cell-type–dependent expression
Rare variantsSporadic variants—require functional validation
Pathway couplingCo-occurs with neighboring nodes on the hub

Naming and prevalence vary by cohort and assay—annotate clinically with COSMIC, ClinVar, OncoKB, and datasheets; research context only.

Assay readouts for PIK3C2B

  • Lipid side: PIP₃/PI(3,4)P₂ probes or PH-domain pulldowns—separate PIK3C2B from class II/III compartments.
  • Biochemistry: IP–kinase or membrane recruitment; co-IP regulatory partners.
  • Downstream: p-AKT Thr308/Ser473, p-S6K, p-4E-BP1—pair totals and inhibitor titrations.
  • Genetics: PTEN/INPP4 status; NGS/ddPCR for lesions and copy number.

PIK3C2B experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for PIK3C2B studies (examples)

  • Tool lines: HEK293T, MCF-7, HCT116, Jurkat, U937—screen by PIK3C2B expression and pathway context (CCLE/DepMap).
  • Combine with neighbors (PIK3C2A, PIK3C2G) via KD/pharmacology to test non-redundancy.
  • Overexpression/rescue in HEK293T supports mechanism and IP workflows.
  • Isogenic/CRISPR models separate pathway dependency from bypass survival.

[2] Cell samples for PIK3C2B Western blot

  • Whole-cell lysates—optimize RIPA/NP-40 per antibody; phosphatase inhibitors for phospho blots.
  • Stimulation: serum starvation ± insulin/EGF or amino-acid withdrawal/refeed as relevant.
  • Controls: siRNA/shRNA, CRISPR KO, or inhibitors to validate band specificity.
  • Loading: BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for PIK3C2B Western blot

  • Matched tumor/adjacent frozen tissues after pathology review.
  • Mouse GEMM or xenografts—mind species antibody cross-reactivity.
  • Primary cells/PDCs when ethically approved.
  • FFPE needs specialized extraction; frozen tissue preferred for phospho work.

[4] Cell samples for PIK3C2B immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-PIK3C2B for complex capture.
  • Endogenous IP: high-expression lines; often ≥1–5×10⁶ cells per IP.
  • Stimulation: ligand or nutrient treatments enrich interactions—pilot time courses.
  • Controls: isotype IgG, empty vector, KD/KO negatives.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous PIK3C2B can differ in dosage, splicing, and compartmentation—in organoids/PDX, record passage, matrix, and drug history.

Quick search presets

PIK3C2BPIK3C2B antibodyPI3K inhibitormTOR inhibitor

PIK3C2B-related antibodies (keyword-biased)

Adds PIK3C2B keyword bias atop the PI3K/Akt/mTOR antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

PIK3C2APIK3C2G

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the PI3K/Akt/mTOR hub for neighboring nodes and assay guidance—cross-check MAPK, RTK, metabolism, and autophagy pages as needed.

PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Metabolism