PI3K/Akt/mTOR · target hub

PPP2R3C

Protein phosphatase 2 regulatory subunit B''gamma (PPP2R3C)

PPP2R3C is a PIP₃ / Akt phosphatase or PP2A regulation on the PI3K/Akt/mTOR axis. PPP2R3C participates in PI3K/Akt/mTOR signaling—see UniProt and primary literature for isoforms and tissue context. It is indexed under "Akt / mTOR-directed phosphatases & PP2A subunits (selected)" on our pathway page for antibodies, inhibitors, and assay guidance.

Back to pathway overviewSearch: PPP2R3CUniProt (G3V5U8)

Research notes

  • Combine with other genes in the same hub block via genetics or pharmacology to test non-redundant roles of PPP2R3C.
  • Annotate PIK3CA/PTEN and upstream RTK–Ras context when reading p-AKT, mTORC1/2, or lipid outputs.
  • Verify antibody clone, phospho-site, and stimulation; include KD/KO or inhibitor controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Expression contextTissue/cell-type–dependent expression
Rare variantsSporadic variants—require functional validation
Pathway couplingCo-occurs with neighboring nodes on the hub

Naming and prevalence vary by cohort and assay—annotate clinically with COSMIC, ClinVar, OncoKB, and datasheets; research context only.

Assay readouts for PPP2R3C

  • Lipid pools: PIP₃, PI(3,4)P₂, or substrates relevant to PPP2R3C.
  • Loss-of-function: CRISPR/siRNA often elevates p-AKT and growth readouts (context-dependent).
  • Tumor specimens: matched tumor/adjacent with mutation/expression annotation.
  • Inhibitor controls: PI3K/mTOR inhibitors to test phenotypic reversibility.

PPP2R3C experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for PPP2R3C studies (examples)

  • Tool lines: MCF-7, U87, PC-3, HEK293T, A549—screen by PPP2R3C expression and pathway context (CCLE/DepMap).
  • Combine with neighbors (PHLPP1, PHLPP2, PPP2CA, PPP2CB) via KD/pharmacology to test non-redundancy.
  • Overexpression/rescue in HEK293T supports mechanism and IP workflows.
  • Isogenic/CRISPR models separate pathway dependency from bypass survival.

[2] Cell samples for PPP2R3C Western blot

  • Whole-cell lysates—optimize RIPA/NP-40 per antibody; phosphatase inhibitors for phospho blots.
  • Stimulation: serum starvation ± insulin/EGF or amino-acid withdrawal/refeed as relevant.
  • Controls: siRNA/shRNA, CRISPR KO, or inhibitors to validate band specificity.
  • Loading: BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for PPP2R3C Western blot

  • Matched tumor/adjacent frozen tissues after pathology review.
  • Mouse GEMM or xenografts—mind species antibody cross-reactivity.
  • Primary cells/PDCs when ethically approved.
  • FFPE needs specialized extraction; frozen tissue preferred for phospho work.

[4] Cell samples for PPP2R3C immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-PPP2R3C for complex capture.
  • Endogenous IP: high-expression lines; often ≥1–5×10⁶ cells per IP.
  • Stimulation: ligand or nutrient treatments enrich interactions—pilot time courses.
  • Controls: isotype IgG, empty vector, KD/KO negatives.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous PPP2R3C can differ in dosage, splicing, and compartmentation—in organoids/PDX, record passage, matrix, and drug history.

Quick search presets

PPP2R3CPPP2R3C antibodyPI3K inhibitormTOR inhibitor

PPP2R3C-related antibodies (keyword-biased)

Adds PPP2R3C keyword bias atop the PI3K/Akt/mTOR antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

PHLPP1PHLPP2PPP2CAPPP2CBPPP2R1APPP2R1BPPP2R2APPP2R2B

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the PI3K/Akt/mTOR hub for neighboring nodes and assay guidance—cross-check MAPK, RTK, metabolism, and autophagy pages as needed.

PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Metabolism