PI3K/Akt/mTOR · target hub

XIAP

E3 ubiquitin-protein ligase XIAP (XIAP)

XIAP is a PDK1–Akt axis or canonical effector on the PI3K/Akt/mTOR axis. Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis (PubMed:11257230, PubMed:11257231, PubMed:… It is indexed under "Canonical Akt substrates & effectors (selected)" on our pathway page for antibodies, inhibitors, and assay guidance.

Back to pathway overviewSearch: XIAPUniProt (P98170)

Research notes

  • Combine with other genes in the same hub block via genetics or pharmacology to test non-redundant roles of XIAP.
  • Annotate PIK3CA/PTEN and upstream RTK–Ras context when reading p-AKT, mTORC1/2, or lipid outputs.
  • Verify antibody clone, phospho-site, and stimulation; include KD/KO or inhibitor controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Expression contextTissue/cell-type–dependent expression
Rare variantsSporadic variants—require functional validation
Pathway couplingCo-occurs with neighboring nodes on the hub

Naming and prevalence vary by cohort and assay—annotate clinically with COSMIC, ClinVar, OncoKB, and datasheets; research context only.

Assay readouts for XIAP

  • Kinase activity: IP–kinase or commercial assays for XIAP where applicable.
  • Phosphorylation: Akt Thr308/Ser473 or substrate-site antibodies—pair totals.
  • Substrate outputs: FOXO/GSK3/BAD phospho-status or localization shifts.
  • Context: PIK3CA/PTEN status; insulin/growth-factor stimulation.

XIAP experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for XIAP studies (examples)

  • Tool lines: HEK293T, MCF-7, HepG2, LNCaP, NIH-3T3—screen by XIAP expression and pathway context (CCLE/DepMap).
  • Combine with neighbors (FOXO1, FOXO3, FOXO4, GSK3A) via KD/pharmacology to test non-redundancy.
  • Overexpression/rescue in HEK293T supports mechanism and IP workflows.
  • Isogenic/CRISPR models separate pathway dependency from bypass survival.

[2] Cell samples for XIAP Western blot

  • Whole-cell lysates—optimize RIPA/NP-40 per antibody; phosphatase inhibitors for phospho blots.
  • Stimulation: serum starvation ± insulin/EGF or amino-acid withdrawal/refeed as relevant.
  • Controls: siRNA/shRNA, CRISPR KO, or inhibitors to validate band specificity.
  • Loading: BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for XIAP Western blot

  • Matched tumor/adjacent frozen tissues after pathology review.
  • Mouse GEMM or xenografts—mind species antibody cross-reactivity.
  • Primary cells/PDCs when ethically approved.
  • FFPE needs specialized extraction; frozen tissue preferred for phospho work.

[4] Cell samples for XIAP immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-XIAP for complex capture.
  • Endogenous IP: high-expression lines; often ≥1–5×10⁶ cells per IP.
  • Stimulation: ligand or nutrient treatments enrich interactions—pilot time courses.
  • Controls: isotype IgG, empty vector, KD/KO negatives.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous XIAP can differ in dosage, splicing, and compartmentation—in organoids/PDX, record passage, matrix, and drug history.

Quick search presets

XIAPXIAP antibodyPI3K inhibitormTOR inhibitor

XIAP-related antibodies (keyword-biased)

Adds XIAP keyword bias atop the PI3K/Akt/mTOR antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

FOXO1FOXO3FOXO4GSK3AGSK3BBADCASP9MDM2

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the PI3K/Akt/mTOR hub for neighboring nodes and assay guidance—cross-check MAPK, RTK, metabolism, and autophagy pages as needed.

PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Metabolism