Transmembrane transport · target hub

AQP12

AQP12 (AQP12)

AQP12 is a Aquaporins on the transmembrane transport axis. Validate function and tissue distribution with UniProt and primary literature. It is indexed under "Aquaporins (AQP)" on our pathway page for antibodies, inhibitors, and assay guidance.

Back to pathway overviewSearch: AQP12UniProt (Q9Y243)

Research notes

  • Combine with other genes in the same hub block via genetics or pharmacology to test non-redundant roles of AQP12.
  • Pair transporter/channel with 13C tracing, Seahorse OCR/ECAR, or metabolomics; nutrient withdrawal/refeed controls.
  • Verify antibody clone, compartment, and stimulation; include KD/KO or inhibitor controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Expression contextTissue/cell-type–dependent expression
Rare variantsSporadic variants—require functional validation
Pathway couplingCo-occurs with neighboring nodes on the hub

Naming and prevalence vary by cohort and assay—annotate clinically with COSMIC, ClinVar, OncoKB, and datasheets; research context only.

Assay readouts for AQP12

  • Water permeability: AQP12 stopped-flow or swelling assays.
  • Glycerol permeability (AQP3/7/9) and cryoprotection.
  • Membrane localization and phospho-regulation (PKA/PKC).
  • Edema/renal concentrating models.

AQP12 experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for AQP12 studies (examples)

  • Tool lines: MDCK, HeLa, HEK293T, Caco-2, A549—screen by AQP12 expression and pathway context (CCLE/DepMap).
  • Combine with neighbors (AQP0, AQP1, AQP2, AQP3) via KD/pharmacology to test non-redundancy.
  • Overexpression/rescue in HEK293T supports mechanism and IP workflows.
  • Isogenic/CRISPR models separate pathway dependency from bypass survival.

[2] Cell samples for AQP12 Western blot

  • Whole-cell lysates—optimize RIPA/NP-40 per antibody; phosphatase inhibitors for phospho blots.
  • Stimulation: serum starvation ± insulin/EGF or amino-acid withdrawal/refeed as relevant.
  • Controls: siRNA/shRNA, CRISPR KO, or inhibitors to validate band specificity.
  • Loading: BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for AQP12 Western blot

  • Matched tumor/adjacent frozen tissues after pathology review.
  • Mouse GEMM or xenografts—mind species antibody cross-reactivity.
  • Primary cells/PDCs when ethically approved.
  • FFPE needs specialized extraction; frozen tissue preferred for phospho work.

[4] Cell samples for AQP12 immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-AQP12 for complex capture.
  • Endogenous IP: high-expression lines; often ≥1–5×10⁶ cells per IP.
  • Stimulation: ligand or nutrient treatments enrich interactions—pilot time courses.
  • Controls: isotype IgG, empty vector, KD/KO negatives.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous AQP12 can differ in dosage, splicing, and compartmentation—in organoids/PDX, record passage, matrix, and drug history.

Quick search presets

AQP12AQP12 antibodyAQP12 ligand/agonisttransmembrane transporter/channel antibody

AQP12-related antibodies (keyword-biased)

Adds AQP12 keyword bias atop the 跨膜转运 antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

AQP0AQP1AQP2AQP3AQP4AQP5AQP6AQP7

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the metabolism & hormone hub for neighboring targets—cross-check PI3K, MAPK, RTK, and metabolism pages as needed.

Transmembrane transport·PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Metabolism