Cellular apoptosis · target hub

APAF1

APAF1 (APAF1)

APAF1 is a Caspase cascade on the Apoptosis signaling pathway axis. Validate with UniProt and literature. Indexed under "Apoptosome" for antibodies and assay guidance.

Back to pathway overviewSearch: APAF1UniProt (Q9Y243)

Research notes

  • Combine with module neighbors to test non-redundant roles of APAF1.
  • Pair phospho/phenotypic readouts with stimulation and inhibitor controls.
  • Validate antibody clone and compartment; include KD/KO controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Loss-of-functionLoss/truncation
CN lossCopy loss
Rare missenseRequire functional validation

Naming and prevalence vary—annotate clinically with authoritative databases.

Assay readouts for APAF1

  • Cleaved APAF1 (WB/IF); initiator vs effector context.
  • PARP1 cleavage as effector activity proxy.
  • z-VAD or caspase inhibitor rescue.
  • Annexin V / PI flow endpoints.

APAF1 experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for APAF1 studies (examples)

  • Tool lines: HeLa, Jurkat, THP-1—screen by APAF1 expression (CCLE/DepMap).
  • Combine with neighbors (CYCS, CASP9, DIABLO, XIAP) via KD/pharmacology.
  • Overexpression/rescue in HEK293T supports mechanism and IP.
  • Isogenic/CRISPR models separate dependency from bypass.

[2] Cell samples for APAF1 Western blot

  • Whole-cell lysates; phosphatase inhibitors for phospho blots.
  • Optimize stimulation time courses per ligand/stress.
  • Controls: KD/KO or inhibitors.
  • BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for APAF1 Western blot

  • Matched tumor/adjacent frozen tissues.
  • Mouse models—mind species cross-reactivity.
  • Primary cells/PDCs when approved.
  • FFPE needs specialized extraction; frozen preferred for phospho.

[4] Cell samples for APAF1 immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-APAF1.
  • Endogenous IP: high-expression lines.
  • Stimulation enriches interactions.
  • Controls: isotype IgG, empty vector, KO.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous APAF1 can differ in dosage, splicing, and compartmentation.

Quick search presets

APAF1APAF1 antibodyapoptosis antibody

APAF1-related antibodies (keyword-biased)

Adds APAF1 keyword bias atop the 凋亡信号通路 antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

CYCSCASP9DIABLOXIAP

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the cellular apoptosis hub for neighboring targets—cross-check PI3K, MAPK, and transmembrane transport pages as needed.

Cellular apoptosis·PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Transmembrane transport