Endocrine & hormone · target hub

IGFBP3

Insulin-like growth factor-binding protein 3 (IGFBP3)

IGFBP3 is a Hormone binding & transport on the endocrine & hormone axis. Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:… It is indexed under "Lactation & GH-axis coupling" on our pathway page for antibodies, inhibitors, and assay guidance.

Back to pathway overviewSearch: IGFBP3UniProt (P17936)

Research notes

  • Combine with other genes in the same hub block via genetics or pharmacology to test non-redundant roles of IGFBP3.
  • For nuclear receptors pair ligands, co-regulators, and reporter assays; for membrane receptors note GPCR/RTK downstream and desensitization.
  • Verify antibody clone, phospho-site, and stimulation; include KD/KO or inhibitor controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Expression contextTissue/cell-type–dependent expression
Rare variantsSporadic variants—require functional validation
Pathway couplingCo-occurs with neighboring nodes on the hub

Naming and prevalence vary by cohort and assay—annotate clinically with COSMIC, ClinVar, OncoKB, and datasheets; research context only.

Assay readouts for IGFBP3

  • Binding: IGFBP3 hormone SPR/radioligand binding.
  • Transport: membrane or nuclear transporter substrate uptake.
  • Binding globulins: free vs total hormone fractions.
  • Tissue distribution: liver/kidney expression & drug-interaction context.

IGFBP3 experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for IGFBP3 studies (examples)

  • Tool lines: HepG2, HEK293T, MCF-7, HeLa, Caco-2—screen by IGFBP3 expression and pathway context (CCLE/DepMap).
  • Combine with neighbors (PRL, PRLR, GH1, GH2) via KD/pharmacology to test non-redundancy.
  • Overexpression/rescue in HEK293T supports mechanism and IP workflows.
  • Isogenic/CRISPR models separate pathway dependency from bypass survival.

[2] Cell samples for IGFBP3 Western blot

  • Whole-cell lysates—optimize RIPA/NP-40 per antibody; phosphatase inhibitors for phospho blots.
  • Stimulation: serum starvation ± insulin/EGF or amino-acid withdrawal/refeed as relevant.
  • Controls: siRNA/shRNA, CRISPR KO, or inhibitors to validate band specificity.
  • Loading: BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for IGFBP3 Western blot

  • Matched tumor/adjacent frozen tissues after pathology review.
  • Mouse GEMM or xenografts—mind species antibody cross-reactivity.
  • Primary cells/PDCs when ethically approved.
  • FFPE needs specialized extraction; frozen tissue preferred for phospho work.

[4] Cell samples for IGFBP3 immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-IGFBP3 for complex capture.
  • Endogenous IP: high-expression lines; often ≥1–5×10⁶ cells per IP.
  • Stimulation: ligand or nutrient treatments enrich interactions—pilot time courses.
  • Controls: isotype IgG, empty vector, KD/KO negatives.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous IGFBP3 can differ in dosage, splicing, and compartmentation—in organoids/PDX, record passage, matrix, and drug history.

Quick search presets

IGFBP3IGFBP3 antibodyIGFBP3 ligand/agonisthormone/receptor antibody

IGFBP3-related antibodies (keyword-biased)

Adds IGFBP3 keyword bias atop the 内分泌和激素 antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

SHBGSERPINA6ALBIGFBP1IGFBP2IGFBP4IGFBP5IGFBP6

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the endocrine & hormone hub for neighboring targets—cross-check PI3K, MAPK, RTK, and metabolism pages as needed.

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