Endocrine & hormone · target hub

MAOA

Amine oxidase [flavin-containing] A (MAOA)

MAOA is a Adrenal medulla catecholamine on the endocrine & hormone axis. Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and periph… It is indexed under "Adrenal medulla catecholamine synthesis & turnover" on our pathway page for antibodies, inhibitors, and assay guidance.

Back to pathway overviewSearch: MAOAUniProt (P21397)

Research notes

  • Combine with other genes in the same hub block via genetics or pharmacology to test non-redundant roles of MAOA.
  • For nuclear receptors pair ligands, co-regulators, and reporter assays; for membrane receptors note GPCR/RTK downstream and desensitization.
  • Verify antibody clone, phospho-site, and stimulation; include KD/KO or inhibitor controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Enzyme activityCatalytic or substrate-channel changes
Steroid outputSteroid profile / metabolite readouts
Rare variantsSporadic variants—require validation

Naming and prevalence vary by cohort and assay—annotate clinically with COSMIC, ClinVar, OncoKB, and datasheets; research context only.

Assay readouts for MAOA

  • Catecholamines: MAOA in NE/epinephrine synthesis or secretion.
  • PC-12 / chromaffin: ACh or glucocorticoid differentiation.
  • Tyrosine hydroxylase / DOPA pathway enzyme activity.
  • Stress models: sympatho-adrenal medullary axis.

MAOA experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for MAOA studies (examples)

  • Tool lines: PC-12, SK-N-SH, HEK293T, AtT-20, H295R—screen by MAOA expression and pathway context (CCLE/DepMap).
  • Combine with neighbors (TH, DDC, DBH, PNMT) via KD/pharmacology to test non-redundancy.
  • Overexpression/rescue in HEK293T supports mechanism and IP workflows.
  • Isogenic/CRISPR models separate pathway dependency from bypass survival.

[2] Cell samples for MAOA Western blot

  • Whole-cell lysates—optimize RIPA/NP-40 per antibody; phosphatase inhibitors for phospho blots.
  • Stimulation: serum starvation ± insulin/EGF or amino-acid withdrawal/refeed as relevant.
  • Controls: siRNA/shRNA, CRISPR KO, or inhibitors to validate band specificity.
  • Loading: BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for MAOA Western blot

  • Matched tumor/adjacent frozen tissues after pathology review.
  • Mouse GEMM or xenografts—mind species antibody cross-reactivity.
  • Primary cells/PDCs when ethically approved.
  • FFPE needs specialized extraction; frozen tissue preferred for phospho work.

[4] Cell samples for MAOA immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-MAOA for complex capture.
  • Endogenous IP: high-expression lines; often ≥1–5×10⁶ cells per IP.
  • Stimulation: ligand or nutrient treatments enrich interactions—pilot time courses.
  • Controls: isotype IgG, empty vector, KD/KO negatives.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous MAOA can differ in dosage, splicing, and compartmentation—in organoids/PDX, record passage, matrix, and drug history.

Quick search presets

MAOAMAOA antibodyMAOA ligand/agonisthormone/receptor antibody

MAOA-related antibodies (keyword-biased)

Adds MAOA keyword bias atop the 内分泌和激素 antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

THDDCDBHPNMTCOMTMAOBSLC6A2SLC6A3

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the endocrine & hormone hub for neighboring targets—cross-check PI3K, MAPK, RTK, and metabolism pages as needed.

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