Cellular cellularAutophagy · target hub

ATG101

ATG101 (ATG101)

ATG101 is a Autophagy initiation on the Cellular autophagy signaling axis. Validate with UniProt and literature. Indexed under "ULK complex" for antibodies and assay guidance.

Back to pathway overviewSearch: ATG101UniProt (Q9Y243)

Research notes

  • Combine with module neighbors to test non-redundant roles of ATG101.
  • Pair phospho/phenotypic readouts with stimulation and inhibitor controls.
  • Validate antibody clone and compartment; include KD/KO controls.

On the pathway hub (sections)

Genetic lesions & expression context (quick reference)

Type / exampleDomain / context (brief)
Expression contextTissue/cell-type–dependent expression
Rare variantsSporadic variants—require validation
Pathway couplingCo-occurs with neighboring nodes

Naming and prevalence vary—annotate clinically with authoritative databases.

Assay readouts for ATG101

  • ULK complex phosphorylation (mTORC1 restraint).
  • p62/SQSTM1 and LC3-II flux.
  • BafA1 to distinguish synthesis vs degradation.
  • Starvation/rapamycin induction.

ATG101 experimental notes

Examples below reflect common literature and public resources (e.g., CCLE, DepMap)—validate genotypes, expression, and passage in your own stocks before committing assays.

[1] Cell lines commonly used for ATG101 studies (examples)

  • Tool lines: HeLa, HEK293T, MEF, U2OS—screen by ATG101 expression (CCLE/DepMap).
  • Combine with neighbors (ULK1, ULK2, RB1CC1, ATG13) via KD/pharmacology.
  • Overexpression/rescue in HEK293T supports mechanism and IP.
  • Isogenic/CRISPR models separate dependency from bypass.

[2] Cell samples for ATG101 Western blot

  • Whole-cell lysates; phosphatase inhibitors for phospho blots.
  • Optimize stimulation time courses per ligand/stress.
  • Controls: KD/KO or inhibitors.
  • BCA normalization; subcellular fractionation when needed.

[3] Tissue samples for ATG101 Western blot

  • Matched tumor/adjacent frozen tissues.
  • Mouse models—mind species cross-reactivity.
  • Primary cells/PDCs when approved.
  • FFPE needs specialized extraction; frozen preferred for phospho.

[4] Cell samples for ATG101 immunoprecipitation

  • Tagged overexpression: HEK293T FLAG/HA-ATG101.
  • Endogenous IP: high-expression lines.
  • Stimulation enriches interactions.
  • Controls: isotype IgG, empty vector, KO.

Human tissues and primary cells require ethics/IRB approval; tumors are heterogeneous—record histotype, site, and preservation conditions.

Bypass & related pathways

Models & genetics note

Overexpression vs endogenous ATG101 can differ in dosage, splicing, and compartmentation.

Quick search presets

ATG101ATG101 antibodyautophagy LC3 antibody

ATG101-related antibodies (keyword-biased)

Adds ATG101 keyword bias atop the 细胞自噬信号通路 antibody pool—if sparse, use presets above or global search.

Neighboring targets (same hub)

ULK1ULK2RB1CC1ATG13

External databases & resources

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-18

Related on this site

See the cellular cellularAutophagy hub for neighboring targets—cross-check PI3K, MAPK, and transmembrane transport pages as needed.

Cellular cellularAutophagy·PI3K/Akt/mTOR·MAPK/ERK·Receptor tyrosine kinase·Transmembrane transport