PI3K/Akt/mTOR · target hub

PIK3CG

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma (p110γ)

PIK3CG encodes class IB PI3K p110γ, commonly partnered with PIK3R5/PIK3R6-encoded regulatory subunits (p101/p84-like modules), preferentially coupling GPCR–Ras inputs to PIP3 production with prominent roles in leukocyte chemotaxis, innate immunity, and inflammatory amplification—often co-shaping PI3K dependence with PIK3CD.

Research notes

  • Strongly aligned with GPCR/chemokine receptor assays—mind overlap with PI3Kδ pharmacology.
  • Useful in chronic inflammation, allergy, and tumor-associated myeloid infiltration models.
  • Activity depends on holoenzyme assembly—pair genetics with isoform-selective probes.

On the pathway hub (sections)

Genetics & holoenzyme context (quick reference)

Type / exampleDomain / context (brief)
p101 / p84 调节亚基Partner levels tune membrane recruitment and Gβγ sensitivity—co-assay PIK3R5/R6
Rare PIK3CG variantsScattered reports—require functional validation
WT + GPCR 轴Classic modeling around chemokine receptors and G-protein inputs
与 PIK3CD 重叠In myeloid cells δ/γ often co-shape chemotaxis/ROS readouts

p110γ is class IB, commonly assembled with PIK3R5/PIK3R6 (p101/p84-like partners)—its lesion spectrum differs from class IA. Annotate with COSMIC, literature, and holoenzyme expression.

Assay readouts for p110γ / PIK3CG

  • GPCR/chemokine receptors: Ca2+ flux, migration, F-actin polarity—use γ-selective/biased inhibitors minding δ overlap.
  • Innate immunity: macrophage polarization, neutrophil respiratory burst, NLRP3-adjacent assays as relevant.
  • Downstream branches (p-AKT, ERK, NF-κB) can run in parallel—annotate stimulus and timing.
  • Holoenzyme: co-IP/WB of PIK3CG + PIK3R5/R6 to verify p101/p84 assembly.

PIK3CG experimental notes

Class IB work must co-track regulatory subunits and G-protein inputs—examples below come from inflammation, allergy, and TAM literature; pilot before large campaigns.

[1] Cell lines commonly used for PIK3CG studies (examples)

  • Myeloid: RAW264.7, THP-1, U937—inflammation/chemotaxis templates; compare PIK3CG KD vs WT.
  • HEK293T PIK3CG + PIK3R5/R6 for Gβγ-coupled receptors (e.g., C5aR co-expression) and IP.
  • Neutrophil-like HL-60 differentiation—annotate differentiation state.
  • Pair with PIK3CD via dual KO or inhibitor matrices for chemotaxis partitioning.
  • Primary murine peritoneal macrophages / human MDMs—physiological but variable.
  • TAM-focused coculture models in select carcinomas—literature-guided.

[2] Cell samples commonly used for PIK3CG (p110γ) Western blot

  • Myeloid lysates across M1/M2 polarization—log cytokines and duration.
  • Transfected HEK293T as positive control/antibody QC.
  • Primary macrophages—LPS priming shifts bands; annotate treatment.
  • Blot alongside PIK3CD to contextualize relative abundance.

[3] Tissue samples commonly used for PIK3CG (p110γ) Western blot

  • Tumor tissues with myeloid infiltrates (lung/liver/GI)—micro-dissect inflamed vs tumor regions.
  • Chronic inflammatory animal tissues (colitis/arthritis models) with ethics approval.
  • Human neutrophils/monocytes from PBMC sorts—record purity/yield.
  • FFPE guidance as other hubs—prefer frozen tissue.

[4] Cell samples commonly used for PIK3CG (p110γ) immunoprecipitation

  • HEK293T FLAG-PIK3CG + HA-PIK3R5/R6 for holoenzyme IP with optional GPCR co-expression.
  • RAW264.7/THP-1 with LPS/chemokine pulses—short windows enrich complexes.
  • Primary macrophages—scale input after enrichment.
  • Controls: isotype IgG, PIK3CG KD, γ-selective pretreatment; optional crosslinkers.

Primary myeloid work needs ethics/biosafety; murine peritoneal macrophages are convenient but differ in receptor profiles from human cells.

Bypass & companion nodes (GPCR / inflammation)

Models & genetics note

p110γ activity depends on Gβγ and regulatory partners—catalytic overexpression alone can overestimate output; human vs murine chemokine receptor profiles differ.

Quick search presets

PIK3CG-related antibodies (keyword-biased)

Adds PIK3CG keyword bias atop the PI3K/Akt/mTOR antibody pool—if sparse, use presets above or global search.

FAQ

This content supports research reagents and pathway education—not medical advice. Annotate mutations, drug indications, and protocols with authoritative databases, datasheets, and institutional oversight.

Last reviewed: 2026-05-03

Related on this site

See the PI3K/Akt/mTOR hub for neighboring nodes and assay guidance—cross-check MAPK, RTK, metabolism, and autophagy pages as needed.